Composite

Part:BBa_K4170054:Design

Designed by: Alexandros Giannopoulos Dimitriou   Group: iGEM22_Thessaloniki_Meta   (2022-09-30)


rhaBAD operon


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 289
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1318
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1586
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

-


Source

The rhaBAD operon was amplified directly from a single colony of E.coli strain DH5a

References